Immunization of cattle against Hyalomma anatolicum anatolicum using larval antigens.

Immunization of rabbits against Hyalomma anatolicum anatolicum using larval and nymphal extracts

The protective capacity of the crude extracts from larval and nymphal stages of Hyalomma anatolicum anatolicum was examined in the New Zealand white rabbits. The rabbits were randomly divided into 3 groups of five animals. The rabbits in groups 1 and 2 were immunized with crude extracts of larval and nymphal antigens, respectively and group 3 was served as control. Following challenge of each r...

Detection of midgut antigens of Hyalomma anatolicum anatolicum tick using SDS-PAGE and Western blot

Ticks are important ectoparasites which are a considerable threat to human beings and to animals all overthe world. Enormous economic losses annually occur in livestock production around the world as a result oftheir existance. One of the ways to control ticks and tick-borne diseases is to introduce resistance to theseectoparasites through immunization. For identification of the putative protec...

Effect of Tick Gut Extract on Protection of Cattle against Hyalomma anatolicum anatolicum

Laboratory evaluation of three strains of the entomopathogenic fungus Metarhizium anisopliae for controlling Hyalomma anatolicum anatolicum and Haemaphysalis punctata

Hyalomma anatolicum anatolicum and Haemaphysalis punctata larval ticks were shown to be susceptible to different strains of entomopathogenic fungi Metarhizium anisopliae under laboratory conditions. To determine the susceptibility of H. anatolicum anatolicum and H. punctata to M. anisopliae, two suspensions of conidia were used (103 and 104 spores/ml). The treatments were conducted by immersing...

Comparative immunopotentiating properties of saponin and incomplete Freund's adjuvant coupled to affinity purified larval antigen of Hyalomma anatolicum anatolicum.

Comparative immunostimulatory properties of saponin and incomplete Freund's adjuvant (IFA) were studied using affinity purified 39 kDa larval antigen of Hyalomma anatolicum anatolicum. Significant antibody response to 39 kDa antigen was detected in the sera of rabbits immunized with both 39 kDa plus saponin and 39 kDa plus IFA in comparison to their corresponding control animals. Insignificant ...